Human Cluster of Differentiation 99 (CD99) ELISA Kit

MIC2X ELISA Kit, MIC2Y ELISA Kit, MIC2 ELISA Kit, Antigen Identified By Monoclonal Antibodies 12E7, F21 And O13 ELISA Kit, E2 antigen ELISA Kit, T-cell surface glycoprotein E2 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD99. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD99. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD99, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD99 in the samples is then determined by comparing the OD of the samples to the standard curve