Rat Mucin 5 Subtype AC (MUC5AC) ELISA Kit

TBM ELISA Kit, MUC5 ELISA Kit, LeB ELISA Kit, Oligomeric Mucus/Gel-Forming ELISA Kit, Mucin 5, Subtypes A And C, Tracheobronchial/Gastric ELISA Kit, Gastric mucin ELISA Kit, Lewis B blood group antigen ELISA Kit, Major airway glycoprotein ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MUC5AC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MUC5AC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MUC5AC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MUC5AC in the samples is then determined by comparing the OD of the samples to the standard curve