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Rat Alanine Aminotransferase (ALT) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alanine Aminotransferase (ALT) .

Product Specifications

Product Name Alternative

GPT ELISA Kit, ALAT ELISA Kit, SGPT ELISA Kit, AAT1 ELISA Kit, GPT1 ELISA Kit, Serum Glutamic Pyruvic Transaminase ELISA Kit, Alanine Transaminase ELISA Kit, Glutamate pyruvate transaminase 1 ELISA Kit, Glutamic--alanine transaminase 1 ELISA Kit

UniProt

P25409

Reactivity

Rat

Field of Research

Pharmacology & Drug Discovery

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

0.26 U/L

References & Citations

Piotr Franciszek Kurzyna et al. Cannabidiolic acid as a modulator of lipid metabolism in the liver of rats with metabolic-associated steatotic liver disease Sci Rep, (2026)

Pubmed id

41792203

Notes

For research use only.

Applications Notes

Standard: 50 U/L. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ALT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ALT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ALT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ALT in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.79-50 U/L

Available Sizes

Frequently Asked Questions

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