Rat Alanine Aminotransferase (ALT) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alanine Aminotransferase (ALT) .
Product Specifications
Product Name Alternative
GPT ELISA Kit, ALAT ELISA Kit, SGPT ELISA Kit, AAT1 ELISA Kit, GPT1 ELISA Kit, Serum Glutamic Pyruvic Transaminase ELISA Kit, Alanine Transaminase ELISA Kit, Glutamate pyruvate transaminase 1 ELISA Kit, Glutamic--alanine transaminase 1 ELISA Kit
UniProt
P25409
Reactivity
Rat
Field of Research
Pharmacology & Drug Discovery
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.26 U/L
References & Citations
Piotr Franciszek Kurzyna et al. Cannabidiolic acid as a modulator of lipid metabolism in the liver of rats with metabolic-associated steatotic liver disease Sci Rep, (2026)
Pubmed id
41792203
Notes
For research use only.
Applications Notes
Standard: 50 U/L. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ALT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ALT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ALT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ALT in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.79-50 U/L
Available Sizes
Frequently Asked Questions
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