Rat Soluble Receptor Activator of Nuclear Factor-kB Ligand (sRANKL) ELISA Kit

CD254 ELISA Kit, TNFSF11 ELISA Kit, ODF ELISA Kit, OPGL ELISA Kit, TRANCE ELISA Kit, HRANKL2 ELISA Kit, SOdf ELISA Kit, Tumor Necrosis Factor (ligand) superfamily Member 11 ELISA Kit, TNF-related activation-induced cytokine ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sRANKL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sRANKL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sRANKL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sRANKL in the samples is then determined by comparing the OD of the samples to the standard curve