Rat Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit

ENPP7 ELISA Kit, E-NPP 7 ELISA Kit, NPP-7 ELISA Kit, Ectonucleotide Pyrophosphatase/Phosphodiesterase 7 ELISA Kit, Sphingomyelin Phosphodiesterase ELISA Kit, Intestinal alkaline sphingomyelinase ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve