Human Interleukin 1 Delta (FIL1d) ELISA Kit

IL1HY1 ELISA Kit, IL36RN ELISA Kit, IL36Ra ELISA Kit, IL1RP3 ELISA Kit, IL1L1 ELISA Kit, IL1F5 ELISA Kit, Interleukin 1 Family, Member 5 ELISA Kit, Interleukin-1 HY1 ELISA Kit, IL-1 Related Protein 3 ELISA Kit, Interleukin 36 Receptor Antagonist ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FIL1d. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FIL1d. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FIL1d, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FIL1d in the samples is then determined by comparing the OD of the samples to the standard curve