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Rat Plasminogen Activator Inhibitor 2 (PAI2) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator Inhibitor 2 (PAI2) .

Product Specifications

Product Name Alternative

SERPINB2 ELISA Kit, PLANH2 ELISA Kit, Serpin Peptidase Inhibitor Clade B Member 2 ELISA Kit, Monocyte Arg-serpin ELISA Kit, Placental plasminogen activator inhibitor ELISA Kit, Serpin B2 ELISA Kit, Urokinase inhibitor ELISA Kit

UniProt

P29524

Reactivity

Rat

Field of Research

Pharmacology & Drug Discovery

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Plasma

Sensitivity

0.58 ng/mL

Notes

For research use only.

Applications Notes

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PAI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PAI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PAI2 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

1.57-100 ng/mL

Available Sizes

Frequently Asked Questions

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