Rat Monocyte Chemotactic Protein 3 (MCP3) ELISA Kit

CCL7 ELISA Kit, FIC ELISA Kit, MARC ELISA Kit, NC28 ELISA Kit, MCP3 ELISA Kit, SCYA7 ELISA Kit, Chemokine C-C-Motif Ligand 7 ELISA Kit, Small Inducible Cytokine A7 ELISA Kit, Monocyte Chemoattractant Protein 3 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MCP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MCP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MCP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MCP3 in the samples is then determined by comparing the OD of the samples to the standard curve