Rat Macrophage Derived Chemokine (MDC) ELISA Kit

CCL22 ELISA Kit, ABCD1 ELISA Kit, DC/B-CK ELISA Kit, SCYA22 ELISA Kit, STCP1 ELISA Kit, Chemokine C-C-Motif Ligand 22 ELISA Kit, Small Inducible Cytokine Subfamily A (Cys-Cys) Member 22 ELISA Kit, Stimulated T-Cell Chemotactic Protein 1 ELISA Kit

Standard: 1500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MDC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MDC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MDC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MDC in the samples is then determined by comparing the OD of the samples to the standard curve