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Mouse CalcitoninGene Related Peptide 1 (CGRP1) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Calcitonin (CT) protein.

Product Specifications

Product Name Alternative

CALCA ELISA Kit, CALC1 ELISA Kit, CGRP-I ELISA Kit, CGRP1 ELISA Kit, KC ELISA Kit, CCP ELISA Kit, PDN-21 ELISA Kit, Katacalcin ELISA Kit, Calcitonin-Related Polypeptide Alpha ELISA Kit, Calcitonin carboxyl-terminal peptide ELISA Kit

Reactivity

Mouse

Field of Research

Metabolism Research, Musculoskeletal & Connective Tissue Research

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

4.68 pg/mL

Notes

For research use only.

Applications Notes

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CGRP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CGRP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CGRP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CGRP1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

15.63-1000 pg/mL

Available Sizes

Frequently Asked Questions

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