Human Vascular Endothelial Growth Factor B (VEGFB) ELISA Kit

Ruonan Zhou 1 2, Yingying Xue 1 2, Ziwei Zhu 1 2, Pingyuan Xu 1 2, Lixuan Shen 1 2, Ziwei Wang 1 2, Yingying Xiang 1 2, Yue Cao 1 2, Xizhong Yu 2, Wenbin Shang VEGF-B is involved in diabetic peripheral neuropathy in patients with type 2 diabetes Growth Factors, (2024)

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VEGFB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VEGFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFB in the samples is then determined by comparing the OD of the samples to the standard curve