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Rabbit Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1 (TGFb1) .

Product Specifications

Product Name Alternative

TGF Beta 1, TGF-B1 ELISA Kit, CED ELISA Kit, DPD1 ELISA Kit, LAP ELISA Kit, Camurati-Engelmann Disease ELISA Kit, Latency-associated peptide ELISA Kit

Reactivity

Rabbit

Field of Research

Stem Cell & Developmental Biology

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids

Sensitivity

6.3 pg/mL

Notes

For research use only.

Applications Notes

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

15.63-1000 pg/mL

Available Sizes

Frequently Asked Questions

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