Rat Epidermal Growth Factor Receptor (EGFR) ELISA Kit

ErbB-1 ELISA Kit, ErbB1 ELISA Kit, ER1 ELISA Kit, ERB-B1 ELISA Kit, MENA ELISA Kit, HER1 ELISA Kit, Erythroblastic Leukemia Viral (v-Erb-B) oncogene Homolog, Avian ELISA Kit, Proto-oncogene c-ErbB-1 ELISA Kit, Receptor tyrosine-protein kinase erbB-1 ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EGFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EGFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EGFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EGFR in the samples is then determined by comparing the OD of the samples to the standard curve