Human Prostaglandin-H2 D-isomerase (PTGDS) ELISA Kit

HPGDS ELISA Kit, GSTS ELISA Kit, H-PGDS ELISA Kit, PDS ELISA Kit, PGD2 ELISA Kit, PGDS ELISA Kit, PGDS2 ELISA Kit, Glutathione S-Transferase Sigma ELISA Kit, Glutathione-requiring prostaglandin D synthase ELISA Kit, Prostaglandin-H2 D-isomerase ELISA Kit

Standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTGDS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTGDS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTGDS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTGDS in the samples is then determined by comparing the OD of the samples to the standard curve