Human Prostaglandin Endoperoxide Synthase 2 (PTGS2/COX-2) ELISA Kit

PGG/HS ELISA Kit, PGHS2 ELISA Kit, PHS2 ELISA Kit, HCox2 ELISA Kit, COX2 ELISA Kit, Cyclooxygenase 2 ELISA Kit, Prostaglandin G/H Synthase And Cyclooxygenase ELISA Kit, Prostaglandin H2 synthase 2 ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve