Rat Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) ELISA Kit

CHR-NA1 ELISA Kit, N-AChRA1 ELISA Kit, NAChRA1 ELISA Kit, ACHRA ELISA Kit, ACHRD ELISA Kit, CMS2A ELISA Kit, FCCMS ELISA Kit, SCCMS ELISA Kit, Acetylcholine receptor subunit alpha ELISA Kit, N-AChR-A1 ELISA Kit, Neuronal Acetylcholine Receptor Alpha 1, Muscle ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CHRNa1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CHRNa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CHRNa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CHRNa1 in the samples is then determined by comparing the OD of the samples to the standard curve