Human Fibromodulin (FMOD) ELISA Kit

SLRR2E ELISA Kit, FM ELISA Kit, Collagen-binding 59 kDa protein ELISA Kit, Keratan sulfate proteoglycan fibromodulin ELISA Kit, KSPG fibromodulin ELISA Kit

Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FMOD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FMOD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FMOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FMOD in the samples is then determined by comparing the OD of the samples to the standard curve