Human Enolase, Non Neuronal (NNE) ELISA Kit

ENO1 ELISA Kit, ENO1L1 ELISA Kit, MPB1 ELISA Kit, PPH ELISA Kit, Alpha Enolase ELISA Kit, Enolase 1 ELISA Kit, Phosphopyruvate hydratase ELISA Kit, Plasminogen-binding protein ELISA Kit, 2-phospho-D-glycerate hydro-lyase ELISA Kit, C-myc promoter-binding ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NNE in the samples is then determined by comparing the OD of the samples to the standard curve