Human Leucine Aminopeptidase (LAP) ELISA Kit

LAPEP, LAP3 ELISA Kit, PEPS ELISA Kit, Peptidase S ELISA Kit, Leucyl Aminopeptidase ELISA Kit, Cytosol aminopeptidase ELISA Kit, Proline aminopeptidase ELISA Kit, Prolyl aminopeptidase ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAP in the samples is then determined by comparing the OD of the samples to the standard curve