Human Delta-Sleep Inducing Peptide (dSIP) ELISA Kit

DSIPI ELISA Kit, TSC22D3 ELISA Kit, DIP ELISA Kit, GILZ ELISA Kit, TSC-22R ELISA Kit, hDIP ELISA Kit, TSC22 Domain Family Protein 3 ELISA Kit, Glucocorticoid-Induced Leucine Zipper ELISA Kit, Delta Sleep Inducing Peptide, Immunoreactor ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human dSIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human dSIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human dSIP in the samples is then determined by comparing the OD of the samples to the standard curve.