Human Placental Protein 13 (PP13) ELISA Kit

LGALS13 ELISA Kit, PLAC8 ELISA Kit, PP13 ELISA Kit, GAL13 ELISA Kit, Galectin 13 ELISA Kit, Galactoside-Binding Soluble Lectin 13 ELISA Kit, Placental tissue protein 13 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PP13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PP13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PP13 in the samples is then determined by comparing the OD of the samples to the standard curve