Human Alpha-2-Plasmin Inhibitor (a2PI) ELISA Kit

SERPINF2 ELISA Kit, AAP ELISA Kit, A2-PI ELISA Kit, API ELISA Kit, PLI ELISA Kit, A2AP ELISA Kit, Alpha 2 Antiplasmin ELISA Kit, Serpin Peptidase Inhibitor Clade F Member 2 ELISA Kit, Pigment Epithelium Derived Factor ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human a2PI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human a2PI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human a2PI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human a2PI in the samples is then determined by comparing the OD of the samples to the standard curve