Human Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) ELISA Kit

CD309 ELISA Kit, FLK1 ELISA Kit, VEGFR ELISA Kit, KDR ELISA Kit, A Type III Receptor Tyrosine Kinase ELISA Kit, Kinase Insert Domain Receptor ELISA Kit, Kinase Insert Domain Receptor ELISA Kit, Fetal Liver Kinase-1 ELISA Kit

Standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VEGFR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VEGFR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve