Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit

BRCA2 ELISA Kit, BRCC2 ELISA Kit, FACD ELISA Kit, FAD ELISA Kit, FAD1 ELISA Kit, FANCD ELISA Kit, FANCD1 ELISA Kit, Breast Cancer 2, Early Onset ELISA Kit, Breast Cancer Type 2 Susceptibility Protein ELISA Kit, Fanconi anemia group D1 protein ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BRCA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BRCA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BRCA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BRCA2 in the samples is then determined by comparing the OD of the samples to the standard curve