Human Perforin 1 (PRF1) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Perforin 1 (PRF1) .
Product Specifications
Product Name Alternative
FLH2 ELISA Kit, HPLH2 ELISA Kit, P1 ELISA Kit, PFP ELISA Kit, Pore Forming Protein ELISA Kit, Cytolysin ELISA Kit, Lymphocyte pore-forming protein ELISA Kit
UniProt
P14222
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
0.64 ng/mL
Notes
For research use only.
Applications Notes
Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRF1 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
1.57-100 ng/mL
Available Sizes
Frequently Asked Questions
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