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Human Mitogen Activated Protein Kinase 14 (MAPK14) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase 14 (MAPK14) .

Product Specifications

Product Name Alternative

CSBP1 ELISA Kit, CSBP2 ELISA Kit, CSPB1 ELISA Kit, EXIP ELISA Kit, Mxi2 ELISA Kit, PRKM14 ELISA Kit, PRKM15 ELISA Kit, RK ELISA Kit, SAPK2A ELISA Kit, P38 ELISA Kit, Mitogen-activated protein kinase p38 alpha ELISA Kit, Cytokine suppressive anti-inflammatory drug-binding protein ELISA Kit

UniProt

Q16539

Reactivity

Human

Field of Research

Cell Biology

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids.

Sensitivity

0.51 ng/mL

Notes

For research use only.

Applications Notes

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAPK14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAPK14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAPK14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAPK14 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

1.57-100 ng/mL

Available Sizes

Frequently Asked Questions

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