Human Heat Shock 70kDa Protein 1A (HSPA1A) ELISA Kit

Małgorzata Kowalska # 1, Marzena Tylicka # 2, Olga Martyna Koper-Lenkiewicz 3, Joanna Kamińska 3, Justyna Dorf 3, Ewa Matuszczak Plasma concentration of MMP-17 is elevated in boys with cryptorchidism and correlates with HSP-70 Sci Rep, (2024)

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSPA1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSPA1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSPA1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSPA1A in the samples is then determined by comparing the OD of the samples to the standard curve