Human Glutathione S Transferase Alpha 1 (GSTa1) ELISA Kit

GSTA1 ELISA Kit, GST2 ELISA Kit, GSTA1-1 ELISA Kit, GTH1 ELISA Kit, GST class-alpha member 1 ELISA Kit, GST HA subunit 1 ELISA Kit, GST-epsilon ELISA Kit, Glutathione S-transferase A1, N-terminally processed ELISA Kit

Standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GSTa1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GSTa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GSTa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GSTa1 in the samples is then determined by comparing the OD of the samples to the standard curve