Human Protein Disulfide Isomerase (PDI) ELISA Kit

P4HB ELISA Kit, ERBA2L ELISA Kit, PDIA1 ELISA Kit, PO4DB ELISA Kit, PROHB, DSI, GIT, PDI, PO4HB, P4Hbeta ELISA Kit, Prolyl 4-Hydroxylase Subunit Beta ELISA Kit, Procollagen-Proline 2-Oxoglutarate 4-Dioxygenase Beta Polypeptide ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDI in the samples is then determined by comparing the OD of the samples to the standard curve