Rat Neuropeptide S (NPS) ELISA Kit

Harish M Kawade 1, Utkarsh P Patil 2, Deepali M Pandhare 3, Nishikant K Subhedar 4, Dadasaheb M Kokare Neuropeptide S system mediates nicotine-induced reward-facilitatory behavior Neuropharmacology ., (2025)

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NPS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NPS in the samples is then determined by comparing the OD of the samples to the standard curve.