Rat Dipeptidyl Peptidase IV (DPP4) ELISA Kit

CD26 ELISA Kit, ADA ELISA Kit, DPPIV ELISA Kit, DPP-IV ELISA Kit, ADABP ELISA Kit, ADCP2 ELISA Kit, TP103 ELISA Kit, Adenosine Deaminase Complexing Protein 2 ELISA Kit, T-cell activation antigen CD26 ELISA Kit

Standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DPP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DPP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DPP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DPP4 in the samples is then determined by comparing the OD of the samples to the standard curve