Rat Elastase 2, Neutrophil (ELA2) ELISA Kit

ELANE ELISA Kit, HLE ELISA Kit, HNE ELISA Kit, NE ELISA Kit, PMN-E ELISA Kit, Neutrophil Elastase ELISA Kit, Medullasin ELISA Kit, Polymorphonuclear Leukocyte Elastase ELISA Kit, Bone Marrow Serine Protease ELISA Kit, Human Leukocyte Elastase ELISA Kit, PMN Elastase ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ELA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ELA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ELA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ELA2 in the samples is then determined by comparing the OD of the samples to the standard curve