Rat Tumor Protein p53 (TP53) ELISA Kit

P53 ELISA Kit, LFS1 ELISA Kit, TRP53 ELISA Kit, Li-Fraumeni Syndrome ELISA Kit, Cellular tumor antigen p53 ELISA Kit, Antigen NY-CO-13 ELISA Kit, Phosphoprotein p53 ELISA Kit, Tumor suppressor p53 ELISA Kit

Standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TP53. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TP53. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TP53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TP53 in the samples is then determined by comparing the OD of the samples to the standard curve