Mouse Monocyte Chemotactic Protein 5 (MCP5) ELISA Kit

CCL12 ELISA Kit, SCYA12 ELISA Kit, Chemokine C-C-Motif Ligand 12 ELISA Kit, MCP-1-related chemokine ELISA Kit, Monocyte chemoattractant protein 5 ELISA Kit, Small-inducible cytokine A12 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MCP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MCP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MCP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MCP5 in the samples is then determined by comparing the OD of the samples to the standard curve