Human Renal Tumor Antigen (RAGE) ELISA Kit

Dominika Kanikowska 1, Alina Kanikowska 2, Zofia Strojny 3, Edyta Kawka 4, Agnieszka Zawada 2, Rafał Rutkowski 4, Monika Litwinowicz 4, Maki Sato 5, Marian Grzymisławski 2, Andrzej Bręborowicz 6, Janusz Witowski 4, Katarzyna Korybalska Assessment of EN-RAGE, sRAGE, and its isoforms: cRAGE, esRAGE in obese patients treated by moderate caloric restriction combined with physical activity conducted in hospital condition Cytokine, 180, 180 (2024)

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RAGE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RAGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RAGE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RAGE in the samples is then determined by comparing the OD of the samples to the standard curve