Human Interleukin 37 (IL37) ELISA Kit

IL1 Zeta, IL1F7 ELISA Kit, IL37 ELISA Kit, FIL1 ELISA Kit, FIL1 (ZETA) ELISA Kit, FIL1Z ELISA Kit, IL-1F7 ELISA Kit, IL-1H4 ELISA Kit, IL-1RP1 ELISA Kit, IL1H4 ELISA Kit, IL1RP1 ELISA Kit, Interleukin 37 ELISA Kit, Interleukin-1-Related Protein ELISA Kit, Interleukin 1 Family, Member 7 ELISA Kit

Standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-37 in the samples is then determined by comparing the OD of the samples to the standard curve