Mouse B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

CXCL13 ELISA Kit, SCYB13 ELISA Kit, BLC ELISA Kit, BCA1 ELISA Kit, BLR1L ELISA Kit, ANGIE ELISA Kit, ANGIE2 ELISA Kit, Chemokine (C-X-C-Motif) ligand 13 ELISA Kit, Small Inducible Cytokine B Subfamily (Cys-X-Cys Motif) Member 13 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BLC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BLC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BLC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BLC1 in the samples is then determined by comparing the OD of the samples to the standard curve