Human Adiponectin (ADP) ELISA Kit

GBP28 ELISA Kit, ApM1 ELISA Kit, AdipoQ ELISA Kit, Acrp30 ELISA Kit, ACDC ELISA Kit, APM1 ELISA Kit, C1Q And Collagen Domain Containing ELISA Kit, Adipocyte Complement-Related Protein Of 30 KDa ELISA Kit, Adipose Most Abundant Gene Transcript 1 ELISA Kit

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Standard: 30 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADP in the samples is then determined by comparing the OD of the samples to the standard curve