Human Osteoprotegerin (OPG) ELISA Kit

TNFRSF11B ELISA Kit, TNFRSF11-B ELISA Kit, OCIF ELISA Kit, OPG ELISA Kit, TR1 ELISA Kit, Tumor Necrosis Factor Receptor Superfamily Member 11b ELISA Kit, Osteoclastogenesis Inhibitory Factor ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OPG in the samples is then determined by comparing the OD of the samples to the standard curve