Mouse Interleukin 6 (IL6) ELISA Kit

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Runhong Zhou 1, Ruifeng Huang 2, Shaofen Zhou 2, Shengsheng Lu 2, Haixing Lin 2, Jingnan Qiu 2, Shuaiqi Ma 2, Jian He Sorbicillinoid HSL-2 inhibits the infection of influenza A virus via interaction with the PPAR-γ/NF-κB pathway J Infect Chemother, (2024)

Standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL6 in the samples is then determined by comparing the OD of the samples to the standard curve