Human Kallikrein 10 (KLK10) ELISA Kit

NES1 ELISA Kit, PRSSL1 ELISA Kit, Kallikrein-Related Peptidase 10 ELISA Kit, Normal epithelial cell-specific 1 ELISA Kit, Protease serine-like 1 ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK10 in the samples is then determined by comparing the OD of the samples to the standard curve.