Human Glucagon (GC) ELISA Kit

GCG ELISA Kit, GLP1 ELISA Kit, GLP2 ELISA Kit, GRPP ELISA Kit, Glicentin-Related Polypeptide ELISA Kit, Glucagen ELISA Kit, Oxyntomodulin ELISA Kit, Incretin hormone ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GC in the samples is then determined by comparing the OD of the samples to the standard curve.