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Mouse Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) .

Product Specifications

Product Name Alternative

Flt1 ELISA Kit, Fms-Related Tyrosine Kinase 1 ELISA Kit, Vascular Permeability Factor Receptor ELISA Kit, Fms-like tyrosine kinase 1 ELISA Kit, Tyrosine-protein kinase receptor FLT ELISA Kit

UniProt

P35969

Reactivity

Mouse

Field of Research

Cardiovascular Research; Stem Cell & Developmental Biology

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma and other biological fluids.

Sensitivity

0.059 ng/mL

Notes

For research use only.

Applications Notes

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGFR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VEGFR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VEGFR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VEGFR1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.16-10 ng/mL

Available Sizes

Frequently Asked Questions

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