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Mouse Interferon Alpha (IFNa) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha (IFNa) .

Product Specifications

Product Name Alternative

IFNα1 ELISA Kit, IFL ELISA Kit, LeIF D ELISA Kit, IFN ELISA Kit, IFN-Alpha ELISA Kit, IFNα13 ELISA Kit, IFN-A ELISA Kit, IFNαP22 ELISA Kit, IFN-Alpha 1b ELISA Kit, Interferon Alpha 1b ELISA Kit, Interferon, Leukocytic ELISA Kit, IFN, Leukocyte ELISA Kit, Interferon alpha-D ELISA Kit

UniProt

P01572

Reactivity

Mouse

Field of Research

Immunology & Inflammation

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

6 pg/mL

References & Citations

Runhong Zhou 1, Ruifeng Huang 2, Shaofen Zhou 2, Shengsheng Lu 2, Haixing Lin 2, Jingnan Qiu 2, Shuaiqi Ma 2, Jian He Sorbicillinoid HSL-2 inhibits the infection of influenza A virus via interaction with the PPAR-γ/NF-κB pathway J Infect Chemother, (2024)

Pubmed id

38942291

Notes

For research use only.

Applications Notes

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IFNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IFNa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IFNa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IFNa in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

15.63-1000 pg/mL

Available Sizes

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