Mouse Interferon Alpha (IFNa) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha (IFNa) .
Product Specifications
Product Name Alternative
IFNα1 ELISA Kit, IFL ELISA Kit, LeIF D ELISA Kit, IFN ELISA Kit, IFN-Alpha ELISA Kit, IFNα13 ELISA Kit, IFN-A ELISA Kit, IFNαP22 ELISA Kit, IFN-Alpha 1b ELISA Kit, Interferon Alpha 1b ELISA Kit, Interferon, Leukocytic ELISA Kit, IFN, Leukocyte ELISA Kit, Interferon alpha-D ELISA Kit
UniProt
P01572
Reactivity
Mouse
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
6 pg/mL
References & Citations
Runhong Zhou 1, Ruifeng Huang 2, Shaofen Zhou 2, Shengsheng Lu 2, Haixing Lin 2, Jingnan Qiu 2, Shuaiqi Ma 2, Jian He Sorbicillinoid HSL-2 inhibits the infection of influenza A virus via interaction with the PPAR-γ/NF-κB pathway J Infect Chemother, (2024)
Pubmed id
38942291
Notes
For research use only.
Applications Notes
Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IFNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IFNa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IFNa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IFNa in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
15.63-1000 pg/mL
Available Sizes
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