Human Factor Related Apoptosis Ligand (FASL) ELISA Kit

CD178 ELISA Kit, CD95L ELISA Kit, CD95-L ELISA Kit, FASLG ELISA Kit, FASLG ELISA Kit, APT1LG1 ELISA Kit, APT1-LG1 ELISA Kit, TNFSF6 ELISA Kit, Fas Antigen Ligand ELISA Kit, TNF Superfamily Member 6 ELISA Kit, Tumor Necrosis Factor (ligand) Superfamily, Member 6 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FASL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FASL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FASL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FASL in the samples is then determined by comparing the OD of the samples to the standard curve