Mouse Amphiregulin (AREG) ELISA Kit

Bingsheng Yang # 1 2 3, Jianwen Su # 1 2, Jichang Wu # 1 2, Zhongwen Wang 1 2, Jin Hu 1 2, Mankai Yang 1 2, Yihuang Lin 1 2, Mingchao Jin 1 2, Xiaochun Bai 4, Bin Yu 1 2, Xianrong Zhang 5 6 Macrophage-derived amphiregulin induces myofibroblast transition in adipogenic lineage precursors near Staphylococcus aureus abscess in bone marrow Nat Commun, (2025)

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AREG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AREG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AREG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AREG in the samples is then determined by comparing the OD of the samples to the standard curve