Anti-ENL Antibody

Mouse Monoclonal Antibody specific to ENL

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

YEATS domain-containing protein 1

Gene Name

MLLT1

NCBI Gene ID

UniProt

Q03111

Cellular Locus

Nucleus.

Host

Mouse

Reactivity

Human

Immunogen

Maltose-binding fusion protein containing ENL amino acids 414-472 (Accession no. NP_005925).

Target Antigen

Protein ENL

Target

ENL

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

Field of Research

Cancer research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA (PubMed:20159561, PubMed:20471948). Specifically rPubMed:20159561, PubMed:20471948, PubMed:27105114}.; Acts as a key chromatin reader in acute myeloid leukemia by rPubMed:28241139, PubMed:28241141}.

Additionnal Information

Immunoblotting: use at 1-5ug/mL. A band of ~62kDa is detected.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human ENL.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

The AF4 and ENL (Eleven nineteen Leukemia) protein families are the most frequent MLL fusion partners accounting for two-thirds of MLL-associated leukemia incidence. The ENL family includes ENL and AF9 and has structural homology to the yeast Anc1 protein. ENL is a nuclear protein that is capable of activating transcription from synthetic reporter genes in both lymphoid and myeloid cells as well as in yeast. Deletion muta-genesis demonstrated that the minimal portion of ENL required for activation of transcription was localized to its C-terminal 90 amino acids. This region is highly conserved between ENL and AF9 and is retained in all Hrx-ENL and Hrx-AF9 fusion proteins. Thus, the leukemogenic contribution and transcriptional activation potential of ENL colocalize to its highly conserved carboxy terminus.