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Goat anti-SPA1 / SIPA1 Antibody

Goat polyclonal antibody to SIPA1

Product Specifications

Product Name Alternative

Anti SIPA1 antibody, anti SPA1 antibody, anti MGC17037 antibody, anti signal-induced proliferation-associated gene 1 antibody, anti p130 SPA-1 antibody, anti GTPase-activating protein Spa-1 antibody, anti signal-induced proliferation-associated 1 antibody, anti MGC102688 antibody, anti signal-induced proliferation-associated gene 1 antibody, anti signal-induced proliferation-associated protein 1 antibody

Reactivity

Human

Target

SPA1 / SIPA1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

ASKQLGSPTADLA

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 1-3 μg/ml

Molecular Weight

112

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 150kDa band observed in Human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112kDa according to NP_694985.28 and NP_006738.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31;272 (44) :28081-8; PMID: 9346962) . Recommended concentration: 1-3 μg/ml. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot.

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

6494

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