4E-T/EIF4ENIF1 Antibody
Goat polyclonal antibody to EIF4ENIF1
Product Specifications
Product Name Alternative
Anti EIF4ENIF1 antibody, anti 4E-T antibody, anti Clast4 antibody, anti FLJ21601 antibody, anti 2610509L04Rik antibody, anti eukaryotic translation initiation factor 4E nuclear import factor 1 antibody, anti eIF4E-transporter antibody, anti FLJ26551 antibody, anti OTTHUMP00000063276 antibody
Reactivity
Human
Target
4E-T / EIF4ENIF1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
AKVISVDELEYRQ
Field of Research
Epigenetics
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.25-0.5 μg/ml, IHC-P: 5-10μg/ml
Molecular Weight
108; 88.2
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10ug/ml.WB: Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ) . Recommended for use at 0.25-0.5 μg/ml
Prediction Reactivity
Bovine, Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
56478
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