Rat Phosphatidylethanolamine Binding Protein 1 (PEBP1) ELISA Kit

PBP ELISA Kit, HCNP ELISA Kit, PEBP ELISA Kit, RKIP ELISA Kit, Prostatic Binding Protein ELISA Kit, Raf Kinase Inhibitory Protein ELISA Kit, Hippocampal Cholinergic Neurostimulating Peptide ELISA Kit, Neuropolypeptide h3 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PEBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PEBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PEBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PEBP1 in the samples is then determined by comparing the OD of the samples to the standard curve